|
Part 4: Can a Rife Generator kill Candida cells? Nope!
Cliquez ici pour la version française
After treating half of our Candida samples with the Rife Generator, we returned to the BRI to check on our cells. Celine prepared the samples and I observed the colonies (top figure) and cells (second figure) under a microscope. Some of the documentation on Rife Generators suggested that the affected cells should lyse (a.k.a. explode) upon treatment. A visual examination failed to detect any differences between the treated or untreated samples nor did we see any cellular debris in the culture media of the treated cells.
This visual examination did not prove that our cells were still alive but it is possible to measure the number of viable cells in the liquid cultures of yeast-shaped Candida (the hyphae are too clumpy for this to work well although we were able to efficiently used them to reinoculate fresh cultures). Celine produced a series of 10, 100, 1000, 10000 and 100000-fold dilutions from the control and Rife-treated liquid cultures. A drop from each dilution was deposited on a fresh petri plate of solid media which was then incubated for one or two days. As you can see in the third figure, dilution series from the treated and untreated samples grew at the same rate thus demonstrating that they contained the same numbers of live cells. She also took 100 microliters of the 100000-fold dilutions and spreaded them on individual petri plates. Under these conditions, each live cell develops into an individual colony and the fourth figure again indicates that there was no significant differences between the two samples.
The final figure comes from a colleague at Universite de Montreal and demonstrates the effect of a clinically-approved antifungal compound called Fluconazone. Anti-microbials work better in a laboratory setting because the target cells are more “accessible” than the ones in infected tissue. In this experiment, the anti-fungal easily killed 99.99% of the same sensitive Candida cells that was used in our tests.
In conclusion, we tested a Rife Generator according to the manufacturer's instructions and failed to observe any indication that it affects the viability of Candida albicans cells.
November 25, 2006 |
Science
|
Comments (5)
 
    
|
